Dear Michael,

thanks for your reaction. We're using a Nikon microscope and infinity
corrected lenses of this manufacturer. The confocal module is the MRC1024
from Biorad. I hope this is helpful to you. Don't hesitate to contact me if
you have any further suggestions.

With best regards,

Kevin Braeckmans

> -----Oorspronkelijk bericht-----
> Van: Confocal Microscopy List
> [mailto:[log in to unmask]]Namens Michael Cammer
> Verzonden: woensdag 17 mei 2000 20:05
> Aan: [log in to unmask]
> Onderwerp: Re: observing microspheres
>
>
> What brand optics are you using?
> Also, resolution is defined by numerical aperture, regardless of specific
> manufacturer's problems.
>
>
> >220µm (!), while in the xy-plane, the diameter was indeed ~15µm.
> When using
> >a 60X NA=1.4 oil immersion lens, the visibility in the z direction was
> >reduced to ~60µm. This suggests that the observed effect has to do with a
> >lens effect of the microsphere itself which apparently depends on the
>
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> *  Michael Cammer * Analytical Imaging Facility * Albert Einstein   *
> *  College of Medicine * 1300 Morris Park Ave. * Bronx, NY  10461   *
> *  (718) 430-2890  *  URL:
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>