BABB is just 1/3 benzyl alcohol and 2/3 benzyl benzoate. To mount
samples in it they must first be thoroughly dehydrated. It clears very
well, but it can cause some changes in tissue morphology. Methyl
salicylate is also worth trying.
Kurt
Goodhouse, Joseph G. wrote:
>
> Could someone please send me a recipe and protocol for BABB mounting
> media.
>
> Regards,
>
> Joe Goodhouse
> Confocal Core Lab Manager
> Dept. of Molecular Biology
> Princeton University
> 609-258-5432
>
> Visit us at _http://www.molbio1.princeton.edu/facility/confocal/_
> <http://www.molbio1.princeton.edu/facility/confocal/>
>
>
--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco
UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517
http://nic.ucsf.edu
phone 415.514.9709
fax 415.514.4300