CONFOCALMICROSCOPY Archives

September 2008

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Subject:
Re: BABB mounting media.
From:
Kurt Thorn <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 22 Sep 2008 12:42:14 -0700
Content-Type:
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BABB is just 1/3 benzyl alcohol and 2/3 benzyl benzoate. To mount 
samples in it they must first be thoroughly dehydrated.  It clears very 
well, but it can cause some changes in tissue morphology.  Methyl 
salicylate is also worth trying.

Kurt

Goodhouse, Joseph G. wrote:
>
> Could someone please send me a recipe and protocol for BABB mounting 
> media.
>
> Regards,
>
> Joe Goodhouse
> Confocal Core Lab Manager
> Dept. of Molecular Biology
> Princeton University
> 609-258-5432
>
> Visit us at _http://www.molbio1.princeton.edu/facility/confocal/_ 
> <http://www.molbio1.princeton.edu/facility/confocal/>  
>
>


-- 
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone 415.514.9709
fax   415.514.4300

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