We had some problems with streptavidin finding endogenous biotin in mouse
tissues. For that reason, I prefer the antibodies
        Kathy



At 04:27 PM 11/16/01 -0600, you wrote:
>Dear all,
>
>I'm in search of flurecent dye for confocal imagine, need your advice.
>
>Probes provide Alexa488 conjugated goat anti-rabbit IgG, there is also
>available of streptavidin conjugated Alexa488. What's the advantage and
>disadvantage between the two choice? What I am going to do is to detect the
>colocalization of two proteins in one type of cell in tissue (mice thyroid
>tissue). Another dye I chose is Alexa568. I have biotin labeled secondary
>antibody. I'd like to try streptavidin-Alexa488 to enhance the signal but
>also afraid this will enhance backgroud (the primary antibody is easy to get
>nonspecific staining). Thanks in advance for help!
>
>All the best!
>
>Kemin
>
>
Kathy Spencer
The Scripps Research Institute
10550 N. Torrey Pines Road
IMM 24
La Jolla, CA 92037
(858) 784-9372
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