In reply to my request for ideas about the molecules which could produce
red autofluorescence (600-620 emission detected after 543 OR 488
excitation), both Glen Macdonald and Martin Wessendorf thought that
lipofucscin was a likely culprit. I have a possible glitch to throw into
this idea, because these are LIVE TISSUE experiments. No fixative. Do you
think lipofuscin would still contribute?
 
        Sorry to not provide this info in the original question...Chip
 
*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
  Chip Montrose
   Johns Hopkins University     tel: 410-955-9681
    Ross 930                      FAX: (410) 955-9677
     720 Rutland Avenue            email: [log in to unmask]
      Baltimore, MD 21205
*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*