Dear Helmut

We have been trying something similar. The problem you face is one of
obtaining a reference signal so you can relate the integrated light
signal to fractional volume. For a reticular membrane structure which
has components much smaller than the PSF you cannot do a simple volume
calculation from 3D reconstruction. I need some more info. before 1
can make any other comments, what are you staining, the membrane or
lumen?

Regards

Mark Cannell


On Thu, 6 Feb 1997 13:35:13 +0100 Helmut Prosch wrote:

> From: Helmut Prosch <[log in to unmask]>
> Date: Thu, 6 Feb 1997 13:35:13 +0100
> Subject: Volume measurement of organelles
> To: [log in to unmask]
>
> Dear List Members
>
> Our institute has recently been equipped with a confocal microscope
> (Zeiss LSM 410) and the Kontron KS400 image analysis system. I am
> working on a project where I have to measure the volume of the Golgi
> Apparatus.
>
> I am presently attempting to achieve this using the whole cell
> fluorescence image. Is it feasible to determine the volume by the
> intensity of the signal? If not, How else might the volume be
> determined?
>
> As a medical student, I have a limited technical background and
would be
> very grateful to any suggestions you might have.
>
> Thank you