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Greetings,

I have been working with someone who is attempting to do double label 
studies using DSRed2-mito and an FITC-based antibody system.  We 
disscovered, using the spectral scan of the Leica SP system, that 
there is significant fluorescence of the DSRed in both the green and 
red wavelengths when we illuminate with a 488 argon line.  Indeed, 
the spectrum appears to have two peaks, of almost equal intensity, in 
response to this excitation.  As a result, we can not separate the 
DsRed Signal from the FITC when the FITC is weak.

At the risk of sounding like a complete novice, has anyone worked out 
a way to deal with this problem? 

Joel


Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
[log in to unmask]  
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs