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Hi, Guy,
as you write, a 50/50 BS is a straight forward solution with no moving
parts a.s.o.
A problem surfaces when one needs to select a wavelength at the edge of
the tuning range. Then, power can become an issue, specifically if there
are further optical elements - EOM, beam expander, spatial filter,
dichroics a.s.o. -on the way to the preparation.
This had been a problem, here, and that's why I went for the more flexible
solution with the lambda/2 plate.
Best,
Johannes
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>
> Just out of curiosity, did you consider using a 50/50 beam splitter? That
> would mean no moving parts, and more stable alignment. I'd have thought
> that modern TiS lasers are powerful enough that both scopes would get
> enough. That way two users could do MP at once - at least if they could
> agree on a wavelength!
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Aust. Centre for Microscopy & Microanalysis, F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Cameron Nowell
> Sent: Thursday, 7 June 2012 8:32 AM
> To: [log in to unmask]
> Subject: Re: two confocal systems on a single multiphoton laser?
>
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>
> Hi Thomas,
>
> We have exactly what you are talking about. We have a single MaiTai
> DeepSee hooked up to two Olympus FV1000 systems (one inverted and one
> upright). The IR induction is pretty simple to set up. Both IR induction
> paths for the microscopes are standard (the inverted one was custom
> built based on Olympus designs for political reasons - the list doesn't
> need to know about this). Infornt of the output of the MaiTai is a
> mirror on a precision motorized stage. When the stage is homed, the
> mirror is in place to bounce the laser into one light path (in our case
> the inverted) and when it is moved to the extreme of its travel the
> laser goes straight into the induction path for the upright scope.
>
> All of this takes up a fair bit of space obviously but ti does allow us
> to have two multiphoton platforms that can also be used for single
> photon imaging as well. We have even had people doing multi on one
> platform and single on the other at the same time.
>
> I am pretty sure that all the big four now offer this type of
> configuration as an option when purchasing it.
>
>
> Cheers
>
> Cam
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research Melbourne - Parkville Branch
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
> Office: +61 3 9341 3158
> Mobile: +61 422882700
> Fax: +61 3 9341 3104
> Facility Website
> Linked In Profile
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Phillips, Thomas E.
> Sent: Thursday, 7 June 2012 7:16 AM
> To: [log in to unmask]
> Subject: two confocal systems on a single multiphoton laser?
>
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>
> Am I correct in assuming that there is no simple practical way to share
> a single multiphoton laser with two confocal systems (e.g., one system
> on an inverted frame for routine work and the other on an upright frame
> dedicated for electrophysiology work)? If someone has actually
> accomplished this, especially in a multi-user environment, I would
> appreciate hearing about it. Thanks, Tom
>
--
P. Johannes Helm
Voice: (+47) 228 51159 (office)
Fax: (+47) 228 51499 (office)
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