Hi, all
Just remember that the 1.49 NA only works with special, high
refractive index liquids.
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At 05:39 PM 3/22/2010, you wrote:
>Hi Charu,
>
>We have A Nikon TIRF module on our Nikon TiE so we have the Apo 60X
>1.49 NA TIRF Objective, I have used this for regular confocal
>imaging with great success.
>
>Best,
>
>Neeraj.
>
>Neeraj V. Gohad, Ph.D.
>Postdoctoral Fellow
>Okeanos Research Group
>Department of Biological Sciences
>132 Long Hall
>Clemson University
>Clemson,SC-29634
>Phone: 864-656-3597
>Fax: 864-656-0435
>
>Website: http://www.clemson.edu/okeanos
>
>Please note my new email address:
><mailto:[log in to unmask]>[log in to unmask]
>
>
>From: Confocal Microscopy List
>[mailto:[log in to unmask]] On Behalf Of Marco Dal Maschio
>Sent: Monday, March 22, 2010 3:56 PM
>To: [log in to unmask]
>Subject: Re: TIRF objective for routine imaging
>
>Dear Charu,
>I had the opportunity to test from the same company 60x 1.45tirf //
>60x VC // 60x 1.49 tirf.
>I remember that probably 60x 1.49 is not plan, related to the flat
>field correction.
>But this objective is currently used by colleagues performing
>tracking and imaging in neuronal cultures
>with optimal image quality. If you want I can send you images
>comparing 60x VC and 60x 1.49 tirf.
>Sorry but no experience about 100x.
>
>best
>Marco
>
>On Mon, Mar 22, 2010 at 10:29 AM, Charu Tanwar
><<mailto:[log in to unmask]>[log in to unmask]> wrote:
>Dear List
>
>Anybody please let me know whether we can use TIRF 100XH (N.A.
>1.49,Working Distance 0.12mm,Coverglass correction 0.13-.20mm - this is a
>special TIRF objective) for routine confocal imaging of bacteria and
>RBC's with
>low flourescence signal.
>Can it be mounted on a confocal microscope from TIRF microscope if both the
>systems are from same company.
>
>Thanks in advance
>
>Charu Tanwar
>Imaging Specialist
>Advanced Instrumentation Research Facility
>Jawaharlal Nehru University
>New Delhi
>India.
>
>
>
>
>
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