CONFOCALMICROSCOPY Archives

May 1994

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Subject:
From:
"Stamatis N. Pagakis" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 3 May 1994 10:47:39 +0100
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On Mon, 2 May 1994, Michael Stanley wrote:
 
>         I am using a Bio-Rad MRC600 to acquire images which I then measure,
> on another computer, using Image-1 software (UIC).  I save these files with
> the bio-rad "BOX" set to 512 x 480 so that they will match up to the
> Image-1 frame store buffer.  The Image-1 normally reads the bio-rad.pic
> files just fine but last week I ran into a problem.  Some of the files that
> I move to the Image-1 machine will not load.  Image-1 shows part of the
> header file in what they call the "annotation file" and the files that will
> not open have missing/scrambled lines.  The bio-rad (Comos) continues to
> open these files just fine.
>
>         I've tried different FTPs and even moving some of the 'bad' files
> on disk, they still won't open.
>
 
Are these new images 16-bit by any chance?  Although all computers
interpret 8-bit data the same way, they don't for 16-bit (or longer) data.
 
 
Stamatis Pagakis
University of Oxford, Dept. of Zoology
South Parks Rd                                 FAX:  +44 (0)865 310447
Oxford OX1 3PS,   United Kingdom               Tel:  +44 (0)865 271194

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