CONFOCALMICROSCOPY Archives

May 1994

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Michael Stanley <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 2 May 1994 16:47:02 -0500
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        I am using a Bio-Rad MRC600 to acquire images which I then measure,
on another computer, using Image-1 software (UIC).  I save these files with
the bio-rad "BOX" set to 512 x 480 so that they will match up to the
Image-1 frame store buffer.  The Image-1 normally reads the bio-rad.pic
files just fine but last week I ran into a problem.  Some of the files that
I move to the Image-1 machine will not load.  Image-1 shows part of the
header file in what they call the "annotation file" and the files that will
not open have missing/scrambled lines.  The bio-rad (Comos) continues to
open these files just fine.
 
        I've tried different FTPs and even moving some of the 'bad' files
on disk, they still won't open.
 
        I can import the images (bio-rad.pic) into photoshop as raw data
and re-save them there as a Tiff and the Image-1 machine will then read
them.  But I have a couple of hundred, or more, and that is rather slow.
I'm also looking for a NIH-Image macro that might do batch conversions for
me but none of the work-arounds address the original problem.  Any ideas?
Anyone have a similar problem?
 
        Any/all help will be greatly appreciated,
C. Michael Stanley
Molecular Cytology Core Facility
Molecular Biology
2 Tucker Hall
University of Missouri-Columbia
Columbia,  MO.    65211
(314) 882-4895
fax= 314-882-0123

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