CONFOCALMICROSCOPY Archives

May 1994

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Subject:
From:
Michael Stanley <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 3 May 1994 10:03:25 -0500
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>On Mon, 2 May 1994, Michael Stanley wrote:
>
>>         I am using a Bio-Rad MRC600 to acquire images which I then measure,
>> on another computer, using Image-1 software (UIC).  I save these files with
>> the bio-rad "BOX" set to 512 x 480 so that they will match up to the
>> Image-1 frame store buffer.  The Image-1 normally reads the bio-rad.pic
>> files just fine but last week I ran into a problem.  Some of the files that
>> I move to the Image-1 machine will not load.  Image-1 shows part of the
>> header file in what they call the "annotation file" and the files that will
>> not open have missing/scrambled lines.  The bio-rad (Comos) continues to
>> open these files just fine.
>>
>>         I've tried different FTPs and even moving some of the 'bad' files
>> on disk, they still won't open.
>>
>
>Are these new images 16-bit by any chance?  Although all computers
>interpret 8-bit data the same way, they don't for 16-bit (or longer) data.
>
>
>Stamatis Pagakis
>University of Oxford, Dept. of Zoology
>South Parks Rd                                 FAX:  +44 (0)865 310447
>Oxford OX1 3PS,   United Kingdom               Tel:  +44 (0)865 271194
 
These images are single slices/images so they are 8-bit.  Thanks.
C. Michael Stanley
Molecular Cytology Core Facility
Molecular Biology
2 Tucker Hall
University of Missouri-Columbia
Columbia,  MO.    65211
(314) 882-4895
fax= 314-882-0123

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