Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I've used the DyLight at greater than 700nm excitation ranges. The
DyLight fluorophore that excites at or around 780nm appears to be far
brighter than the IRDye800 by LiCor. I didn't make any exact
measurements, but the DyLight flourophores appeared brighter by eye.
The traditional wavelength ranges in the visible region, what I have
seen because I imaged these for a commercial enterprise, appeared to
mimic the brightness of Alexa dyes, but it could have been the sample,
concentration, etc.
In any case, I plan to abandon the LiCor dyes for the DyLight in
excitation wavelengths greater than 700nm. I didn't use the buffers.
Jerry
Kathryn Spencer wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Has anyone heard of these fluors? How are they?
> Kathy
>
>
>
> *Try our New DyLight^TM Fluorophore Conjugates at No Risk!**
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> *
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> with the intense fluorescence of DyLight^TM dyes - a winning combination!*
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> offer the sensitivity and reproducibility of KPL's antibodies with the
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> analysis in fluorescence microscopy, flow cytometry, Western blotting,
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> **
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> **
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> **
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> */Fluorescent Detection on Western Blots/*//
>
> /DyLight 549 Goat Anti-Rabbit and DyLight 649 Goat Anti-Mouse
> secondary antibody conjugates provide low background and high signal
> in two-color Western blot detection of tubulin and TNF(alpha).
> Membranes were imaged with the Typhoon 9410. Dyes are artificially
> colorized to maximize contrast./**
>
> **
>
> KPL couples three of the most common DyLight dyes, DyLight 488
> <http://pull.t2mr3.com/p/6171-C964/12979502/clickto2_Catalog_ID=17-Category_ID=482.html>,
> 549
> <http://pull.t2mr3.com/p/6171-98E4/12979510/clickto3_Catalog_ID=17-Category_ID=483.html>
> and 649
> <http://pull.t2mr3.com/p/6171-C856/12989380/clickto4_Catalog_ID=17-Category_ID=484.html>
> that have similar excitation and emission spectra to the dyes seen in
> the table below:
>
> * *
>
> *Color*
>
>
>
> *Ex/Em **l nm*
>
>
>
> *Replaces*
>
> DyLight 488 green
> <http://pull.t2mr3.com/p/6171-9F97/12979259/clickto5_Catalog_ID=17-Category_ID=482.html>
>
>
>
>
> 493/518
>
>
>
> Alexa 488, FITC
>
> DyLight 549 yellow
> <http://pull.t2mr3.com/p/6171-6817/12979263/clickto6_Catalog_ID=17-Category_ID=483.html>
>
>
>
> 550/568
>
>
>
> Alexa 546, Alexa 555, TRITC, Cy3
>
> DyLight 649 red
> <http://pull.t2mr3.com/p/6171-6A64/12979526/clickto7_Catalog_ID=17-Category_ID=484.html>
>
>
>
> 646/674
>
>
>
> Alexa 647, Cy5
>
> *DyLight Performance Comparable to Alexa*
>
> The performance of DyLight dyes is comparable to Alexa^® dyes and
> superior to conventional Cy^TM dyes and fluorescein. Learn more
> <http://pull.t2mr3.com/p/6171-5AD7/12989396/clickto8_7564027b7c117373-jb=ffcf14-t=.html>
> about our growing line of DyLight 488, DyLight 549 and DyLight 649
> antibody and streptavidin conjugates.
>
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> Take advantage of this limited-time offer and see for yourself!
> Evaluate a conjugate of your choice at no charge, a $75 value! KPL
> offers trial sizes of our anti-mouse, rabbit, human and rat conjugates
> and streptavidin. Click here
> <http://pull.t2mr3.com/p/6171-A557/12978653/clickto10_564027b7c117373-jb=ffcf14-t=.html>
> for more details.
>
> **
>
> *Introducing Quality Immunoassay Buffers*
>
> Take the time and effort out of your buffer preparation with KPL’s new
> line of buffers
> <http://pull.t2mr3.com/p/6171-94D6/12978661/clickto11_atalog_ID=17-Category_ID=486.html>.
> These convenient liquid concentrates eliminate the need for
> time-consuming buffer preparation. We offer a variety of buffers
> designed for use in general immunoassay applications such as Western
> blotting, gel electrophoresis, ELISA and sample preparation.
>
> Experience these advantages when using KPL’s line of buffers:
>
> * Defined pH range eliminates need to adjust pH after dilution
> * Carefully controlled conductivity ensures substantial buffering
> capacity
> * Convenient “dilute and use” format reduces potential for
> assay-to-assay variation
> * Pretested buffers produced in our ISO 9001:2000 facility provide
> consistent performance
>
> Our line of buffers is growing so be sure to check back!
>
> *Stay updated on the latest products from KPL*
>
> Visit www.kpl.com
> <http://pull.t2mr3.com/p/6171-3EE4/12979538/clickto13_d610c74771c74-feff15717d6707.html>
> for information, products and promotions associated with protein
> detection applications. While you are there take a minute to give us
> your contact information
> <http://pull.t2mr3.com/p/6171-0966/12979542/clickto14_egistration-registration.cfm.html>
> and receive a retractable Sharpie lab marker at no charge! Just write
> “DyLight email” in the Comments section.
>
> **
>
>
>
> DyLight is a trademark of Thermo Fisher Scientific Inc. and its
> subsidiaries.
> Alexa is a registered trademark of Invitrogen, Inc.
> Cy is a trademark of GE Healthcare.
>
>
>
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Jerry (Gerald) Sedgewick
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