Dear Martin and others,

sorry, I see that I should be more clear. Indeed the question is if
someone has experience in replacing a color camera by a combination of
multi-LED illumination and a monochrome camera.

We would prefer a monochrome camera, because this could then also be
used for efficient fluorescence imaging and we would not have to switch
between a color and a monochrome camera.

For fluorescence we are using regular epi-illumination, for LED pseudo
color we would use transmission illumination.

Thanks, jens

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Martin Wessendorf
Sent: Wednesday, October 28, 2009 3:46 PM
To: [log in to unmask]
Subject: Re: question: camera for macroscope

Dear Jens--

Rietdorf, Jens wrote:

> we are looking for suitable illumination & camera/s for our Leica Z6
> MacroFluo. It is used for both fluorescence and absorption contrast
> samples.
> 
> Has anybody out there experience with LED illumination in combination
> with a monochrome camera to generate 'true color' images?

Clever idea!  Sounds as if it'd work nicely for transmitted illumination

and tinctorial staining.  However, unless I'm misunderstanding you, I 
don't think it would work as well for fluorescence, since the emission 
wavelengths observed would be a function of the molecule rather than the

excitation wavelength.

Good luck--

Martin Wessendorf
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [log in to unmask]