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December 2011

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From:
"MODEL, MICHAEL" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 9 Dec 2011 06:18:51 -0500
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20-30 microns in water. I doubt such lenses exist but who knows... Thanks!

Mike

________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Martin Wessendorf [[log in to unmask]]
Sent: Thursday, December 08, 2011 9:20 PM
To: [log in to unmask]
Subject: Re: small and variable working distance

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Dear Mike--

How close do you need to get and what kind of resolution do you need?
And in what medium?  Air?  Water?  Oil?

Good luck!

Martin

On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:

> Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Martin Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [log in to unmask]
> Subject: Re: small and variable working distance
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
>> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might
> help me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf

--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
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