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Matt:
 
Reinstalling the program do that trick to you, now you are working in the "normal" way, the only thing you have to do, is change the filter cube for your dapi to the position where the system is making the confocal imaging, that might fix it.
 
Best regards and happiness
 
Gabriel OH
 
> Date: Wed, 24 Jul 2013 17:31:46 +0100
> From: [log in to unmask]
> Subject: Re: Pseudo DAPI on an old 510
> To: [log in to unmask]
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> 
> Hi Keng,
> 
> I suspect we have an older version of the 510 here but this is our  
> single track config for pseudo DAPI:
> 
> Specimen>NT 80/20, Mirror, Mirror, BP 390-495, Ch2.
> 
> We use one of the sliding filter cube turret positions as the epi- 
> fluorescence shutter.  The pseudo DAPI setting just moves the slider  
> to the DAPI position.
> 
> I can't really explain why its not saving the light path and  
> microscope settings that you've selected, unless for some reason there  
> are write issues to the folder where configs are saved to on the hard  
> disk?  I only say that because you've just had it replaced..
> 
> Thanks,
> 
> Matt
> 
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