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Jennifer, There are couple of options as far as our recent study on this
subject. There are two programs i.e., Metamorph and Image Pro-Plus can
perform this function with additional features including providing you a
correlation coefficient (Pearsons coefficient), cololaization coeficient as
well as percent colocalization. Both are in principle following the same
algorithm published in this article (reference at the bottom) and in
Metamorph you have the option to use both thresholded and unthresholded
images. In general, there are some advantages and disadvantages with both
the programs.

Should you need further information on this please let me know.

Shiv

Dr. Mayandi Sivaguru, Ph.D.,
Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2, Tucker Hall
University of Missouri
Columbia, MO 65211-7400

Voice: 1-573-882-4895
Fax: 1-573-882-0123

www.biotech.missouri.edu/mcc/

Manders EMM, Verbeek FJ and Aten A (1992) Measurement of co-localization of
objects in dual-colour confocal images. Journal of Microscopy 169: 375-382.