Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Stan,
I've had the experience of general cell staining with plain old FITC in a 
buffer.  Since the material was stained with unconjugated rhodamine B, you 
might give unconjugated FITC a try.

Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
----- Original Message ----- 
From: "George McNamara" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, January 30, 2008 8:51 PM
Subject: Re: Green fluorescing counterstain?


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Reflected light confocal microscopy is much brighter than using a 
> fluorescent dye.
>
>
>
>
> At 07:02 PM 1/30/2008, you wrote:
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Dear confocalists,
>>do you have suggestions for a general counterstain that would fluoresce in
>>green?
>>I am doing 3D imaging of large number of pollen samples, and would like to
>>increase the signal levels so that my acquisition times become more
>>reasonable.
>>
>>The pollen has been processed (cytoplasm removed), so I am trying to stain
>>the shell (exine/intine) and am not interested in the cytoplasm.
>>
>>Rhodamine B staining worked fine, but the emission is in red. I am after
>>shorter wavelength signal for best resolution.
>>
>>Thank you!
>>
>>
>>Stan Vitha
>
>
>
>
>
>
> George McNamara, Ph.D.
> University of Miami, Miller School of Medicine
> Image Core
> Miami, FL 33010
> [log in to unmask]
> [log in to unmask]
> 305-243-8436 office
> http://home.earthlink.net/~pubspectra/
> http://home.earthlink.net/~geomcnamara/
> http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc (see 
> Analytical Imaging Core Facility)
>