Hi Esteban,

We too face the same problem with 710 meta and Zeiss has no solution for it.
We have to do this one by one. Even with ImageJ, it cannot be done
automatically. Though, I tried with ImageTrak in the Mac, I could not do the
RGB pseudo coloring simultaneously. I guess, we need to ask Zeiss to develop
a plugin for this problem.

Roshma.



On Fri, Aug 6, 2010 at 9:59 PM, Craig Brideau <[log in to unmask]>wrote:

> Our ImageTrak software can handle spectral data and display in RGB.
>
> http://www.ucalgary.ca/styslab/imagetrak
>
> It is Mac only though.
>
> Craig
>
>
> On Fri, Aug 6, 2010 at 1:21 PM, G. Esteban Fernandez
> <[log in to unmask]> wrote:
> > I have 4D (xyz + lambda/spectral) stacks from a Zeiss 710 confocal that I
> > need to render in 3D, with the lambda dimension pseudocolored in RGB.
> > Zeiss AIM/LSM and ZEN software do not render 3D volumes of lambda-colored
> > z-stacks or even export series of lambda-colored planes to TIFF (I'd have
> to
> > do 'em one by one) so I'm going to color each channel according to
> > wavelength using ImageJ's Image5D format.  Might there be an ImageJ
> plugin
> > or other software that reads the metadata and does this automatically?
> >
> > Thanks,
> > Esteban
>