CONFOCALMICROSCOPY Archives

August 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 28 Aug 1997 19:22:38 +1000
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Harvey Karten wrote:
>
>You can use NIH-Image to generate z-projections,

and a lot more.

The point of the Iain's additions to NIH-Image was perhaps
not made clear enough in the posting from Pal & Matthew.

It does something quite different from other 3D add-ins
in that by carrying out hyperbolic interpolation it
locates the true position of a surface to a much higher
level of accuracy than than the focus motor step size.

It is, therefore, solely for confocal applications - it
is not in any sense a general-purpose surface extraction
program.  But if you are profiling surfaces with a
confocal microscope it gives you both amazing accuracy
and a very attractive picture!

Iain originally compiled it (a couple of years ago) for
68xxx processors only - it has now been recompiled for
PowerMac users.
Like all other versions of NIH Image it is freeware.

                                        Guy Cox



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