In reply to Johan Wasselius regarding organelle probes,

Try Molecular probes, they have a variety of organelle specific probes - including antibodies.

The following are probes are described in the molecular probes on-line handbook (www.probes.com), search their web site rather than for a specific product to get the best information.

Golgi probes: A newly available antibody (anti-human golgin-97, product number A-21270).

ER probes: ER identification in fixed cells using Alexa Fluor conjugates of concanavalin A (con A).

Lysosomes: LysoTracker Probes - retain staining after fixation with aldehydes. 

Lysosomes/endosomes: Endocytic markers such as fluorescently labelled dextran or latex beads. Many of these probes retain their fluorescence after fixation.

Good luck, Alan Hibbs.

BIOCON, specialists in confocal microscopy
7 Walhalla Drive, Ringwood East VIC 3135 Australia      phone: 9876 9822
Dr. Alan R. Hibbs


-----Original Message-----
From:   Johan Wasselius [SMTP:[log in to unmask]]
Sent:   Saturday, February 19, 2000 9:01 AM
To:     [log in to unmask]
Subject:        ER, Golgi, Lysosome and Endosome-lebeling

Dear fellow confocalists,

In our lab we have for some time studied a group of cysteine-protease
inhibitors. We are now looking at their intracellular distribution in
cultured human neuroblastoma cell lines (LA1-5s and CHP-234) using
immunohistochemistry and confocal microscopy. To really be able to say
something about its intracellular distribution we would like to co-label our
protease inhibitor with the ER, Golgi, Lysosones and Endosomes respectively.

We use immunohistochemistry on formaline fixated cells to detect the protease
inhibitor, so we are looking for antibodies against the mentioned organelles,
but also other markers that may be combined with immunohistochemistry and
examined by confocal microscopy. Labeling of the organelles is new territory
for me, so suggestions from you guys would be of tremendous help.

Cheers,

Johan


Johan Wasselius
Wallenberg Retina Center
Lund University Hospital
Lund, Sweden

e-mail: [log in to unmask]