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Hi Karl,

Fm 4-64 emission undergoes a "blue-shift" when it binds to membranes.  Although
the emission peak in chloroform is 750 nm, the 640 nm range is usually where the
peak is for the membrane bound dye.

I hope that this helps.

--
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Beth Browne, Ph.D.
Technical Assistance Specialist

Molecular Probes Inc
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Karl Garsha wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anybody have information pertaining to the absorbtion spectra of
> polyester tissue culture membranes?  Specifically, I'm looking at cell
> membranes labeled with FM 4-64 on cells cultured on Corning-Costar
> Transwell Clear membranes.  The emmission spectra of FM 4-64 appears to be
> attenuated above 650nm in this instance, so I am wondering if the polyester
> membranes are quenching fluorescence from FM 4-64. The emmission spectra of
> FM 4-64 is supposed to peak at about 750nm, I'm getting peak emmission at
> 650nm and then a drop to baseline. Thanks in advance.
> -Karl G.
>
> _______________________________________________
> Karl Garsha
> Light Microscopy Specialist
> Imaging Technology Group
> Beckman Institute for Advanced Science and Technology
> University of Illiniois at Urbana-Champaign
> 405 North Mathews Avenue
> Urbana, IL 61801
> Room B650J
> Tel: (217) 244-6292
> Fax: (217) 244-6219
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