Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Marco,
In response to your inquiry and comments from others:
As it turns out, George McNamara and myself have been working on just such a
resource for some time. We currently have about 800 spectra in the database,
mostly fluorescent molecules, but also some of lamps, filters and mirrors.
The current url is: http://www.mcb.arizona.edu/ipc/fret/ The site has some
"logistical" flaws that need to be worked out, but it is currrently
functional. There is a turorial, which is useful to appreciate what the
site has to offer, or you can go directly to the graphing/data page. Flash
ver. 6.0 (free download from Macromedia.com) is needed to make the site
work, and the current version loads slowly, so be patient.
The first page simply lets you view the spectral curves for the items in
the database and a Notes page provides additional info. The calculations
button takes you to another page where you have the ability to derive some
indicies of fluorescent efficiencies by selecting any combination of dye,
light source, ex
filter, em filter and dichroic mirror. The indices are defined at
http://www.mcb.arizona.edu/ipc/fret/definitions.htm.
Anyone using the site should feel free to make comments or suggestions as
you see fit. Our intent
it to keep the site as up-to-date as possible, so spectral data on new
molecules is very welcome. Please contact George ([log in to unmask]) or
myself ([log in to unmask]) to discuss data formatting and content
issues. Also, if there is anyone with expertise in Flash and/or MS Access
who would like to be involved in this site, please feel free to get in
touch. We could use some help with database management and additional
interactive features on the site.
Regards,
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-626-8469
FAX 520-621-3709
----- Original Message -----
From: "Marco Prado" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, April 28, 2005 9:44 AM
Subject: Re: Online fluorescence protein resource
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I think it would be particularly useful if a database containing fusion
> proteins with EGFP, EYFP and others is available to the research
> community. People could send information on their lab's constructs with
> references on published constructs.
>
> Dr. Marco Antonio M. Prado
> Associate Professor
> Department of Pharmacology, ICB
> UFMG, Av. Antonio Carlos 6627 Belo Horizonte, MG
> 31275-120, Brazil
> tel 55 31 34992718
> fax 55 31 34992695
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Michael J. Herron
> Sent: Thursday, April 28, 2005 1:24 PM
> To: [log in to unmask]
> Subject: Re: Online fluorescence protein resource
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> please do.
>
>
> On Apr 28, 2005, at 10:25 AM, simon walker (BI) wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Presumably Jeff is after a database specifically for the latest
>> proteins
>> from the likes of R Tsien et al. I too would like to know if there is
>> an online resource for GFP variants or new fluorescent proteins with
>> details such as excitation/emission profiles, references to their
>> design
>> and use and where you can get hold of them. Some kind of user input
>> would be ideal so that those using these things in the real world can
>> cut though some of the commercial/publication spin.
>>
>> Perhaps I need to start one..
>>
>> Simon
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[log in to unmask]]
>> On
>> Behalf Of Debbie Hardie
>> Sent: 28 April 2005 15:53
>> To: [log in to unmask]
>> Subject: Re: online fluorescence protein resource
>>
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> try
>> http://www.zeiss.de/C12567BE0045ACF1/Inhalt-Frame/
>> E840CF3ECD8AF76EC1256F
>> A500346448?OpenDocument&Highlight=fluorochrome%20database
>>
>> Debbie Hardie
>> Research Fellow
>> MRC Centre for Immune Regulation
>> Institute of Biomedical Research Floor 4
>> University of Birmingham
>> Birmingham
>> B15 2TT
>>
>> 0121 414 6970
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
> [mailto:[log in to unmask]]On
>> Behalf Of Reece, Jeff (NIH/NIEHS)
>> Sent: 28 April 2005 15:13
>> To: [log in to unmask]
>> Subject: online fluorescence protein resource
>>
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Does anyone know of an online resource that maintains a list of
>> currently available fluorescent proteins?
>>
>> Or, if not, please let the list know what are the current best
> versions
>> of fluorescent proteins (all colors welcome)?
>>
>>
>> Cheers,
>> Jeff M. Reece
>> Biomedical Engineer
>> Confocal Microscopy Center
>> National Institute of Environmental Health Sciences (NIEHS)
>> 111 Alexander Drive, Bldg, 101, Rm. F219
>> P.O. Box 12233, MD F2-02
>> Research Triangle Park, NC 27709
>> (919) 541-0311
>> [log in to unmask]
>>
>>
> ---
>
> Michael J. Herron, U of MN, Dept. of Entomology
> [log in to unmask]
> 612-624-3688 (office) 612-625-5299 (FAX)
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