Steffen Dietzel wrote:
>
> --- James Pawley <[log in to unmask]> wrote:
> > Watch out for severe spherical aberration if you use coverslips that are
> > thinner than those recommended for the objective  you are  using (usually
> > 0.17 mm).
> >
> > Jim Pawley
>
> I never really understood that one, at least not for oil immersion lenses
> (Although I take care to get the right cover glasses anyway.):
> Shouldn't the immersion oil and the cover glass have the same refractive index
> so that the exact thickness of the cover glass shouldn't matter, at least in
> theory?

Neither do I understand it, and I would be very interested in an
explanation. However, concerning the Bioptechs coated coverslips I
talked to somebody who had problems (which in the end he solved) using
them with laser tweezers. It seems as if the indium coating creates some
aberrations.

Joachim



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Joachim Walter, Dipl. Phys.
Institut für Anthropologie und Humangenetik der LMU München
AG Cremer
Richard-Wagner-Straße 10/I
D-80333 München               Tel. +49 - 89-2180-6713
Germany                       Fax. +49 - 89-2180-6719
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