Thanks Joe,

Very useful info. Was wondering if you have ever tried a
homobifunctional succinimidyl crosslinkers such as DSS or DSSD. I
have found the latter helpful as a pre-fix for microtubules and was
wondering if this might provide some modest wet cell fixation without
compromising GFP fluorescence.

Mario



>We have been employing GFP in confocal imaging here for a number of years in
>a wide variety of specimens ( fly embryos,  yeast and bacterial cells and a
>wide variety of mammalian cells).  It is a wonderful fluorescent tag for
>tracing gene expression and molecular transport, and cell shape changes just
>to mention a few of its uses.  But it does and can have problems in any
>system from over expression, under expression, bleaching and leaching.  So I
>thought I would pass on a few tips about its use for people who are just
>beginning to employ it in their work.
>
......


Mario M. Moronne, Ph.D.
NanoMed Technologies, Inc.
1561 Posen Ave
Berkeley, CA
94706