Hi Irina,
 I just read your email about sealing glasses with the "base coat".
Could you give me the precise name/brand of the stuff you use?
Thanks very much!
Regards,
Katharina
PS: Sorry to all users, I wanted to send this to Irina´s address only, but
something seems to be wrong with her email address and my  message was not
delievered.
Irina Solovei wrote:

> Dear all,
>
> Coming back to the discussion about sealing of coverslips I would like to
> mention two points.
>
> (1) We use for our slides not a nail polish but other cosmetic stuff, so
> called "base coat". It hardens quicker then a nail polish, it is
> colourless, and does not course bleaching of GFP in fixed cells - at least
> it is not a problem to scan them with CLSM.
>
> (2) In connection to the nail polish sealing, I recall my private
> discussions with microscopists about harmful effect of nailpolish on
> objective lenses. Do we really risk our very expensive lenses using sealed
> preps? Does anybody can comment on it?
>
> Thanks,
>
> Irina
>
> ===========================
>           Dr.I.Solovei
>
>    Institute of Anthropology
>        and Humangetics
>    University of Munich (LMU)
>
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