CONFOCALMICROSCOPY Archives

March 2000

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Visible DNA stain
From:
Alan Hibbs <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 17 Mar 2000 11:15:05 +1100
Content-Type:
text/plain
Parts/Attachments:
text/plain (12 lines) 
In reply to Don,

Propidium Iodide stains DNA much more strongly than RNA (due to the large amount of dsDNA compared to dsRNA in the cell) and so is most useful as a DNA stain even without RNAase treatment as suggested by Jonathan Bard. Otherwise the RNAase treatment does work very well to remove any RNA staining.

Alan.

BIOCON, specialists in confocal microscopy
7 Walhalla Drive, Ringwood East VIC 3135 Australia      phone: 61 3 9876 9822
Dr. Alan R. Hibbs

ATOM RSS1 RSS2