--- Alan Hibbs <[log in to unmask]> schrieb:
> In reply to Don,
>
> Propidium Iodide stains DNA much more strongly than RNA (due to the large
> amount of dsDNA compared to dsRNA in the cell) and so is most useful as a DNA
> stain even without RNAase treatment as suggested by Jonathan Bard. Otherwise
> the RNAase treatment does work very well to remove any RNA staining.
>
> Alan.

That probably depends very much on the cell type. I once tried to stain
Drosophila nuclei with several non-DNA-specific dyes, including PI, and the
result was that I got black holes (=nuclei) in a sea of bright cytoplasm. After
RNase treatment the picture reverted.
Alan, which cell type did you use?

Steffen

=====
Dr. Steffen Dietzel
Dept. of Cell and Structural Biology
University of Illinois at Urbana-Champaign
601 S. Goodwin Ave.
Urbana, IL 61801
Phone: +1/217/333-8372  Fax: +1/217/244-1648
e-mail: [log in to unmask]  Web:  http://www.life.uiuc.edu/belmont/dietzel

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