--- waldir Caldeira <[log in to unmask]> schrieb:
>     Hello.
>
>     I work with  insects gut epithelium  and   I have had the same
> problem with PI, ToTo and BoBo, that stain all  nuclei and cytoplasm.
>     Would it be possible for someone to tell me which concentration of
> RNAase I should use in order to avoid inespecific staining?
>     Thanks.

For imaginal disks I used 0.1 mg/ml in PBS for ~ 90 min at 37 degree (after 5
min in 1%Triton in PBS). If you test a new tissue, I would try e.g. 30 min, 60
min, 90 min and 120 min and just check the PI-stained result under the
microscope. Hope this helps

Steffen

=====
Dr. Steffen Dietzel
Dept. of Cell and Structural Biology
University of Illinois at Urbana-Champaign
601 S. Goodwin Ave.
Urbana, IL 61801
Phone: +1/217/333-8372  Fax: +1/217/244-1648
e-mail: [log in to unmask]  Web:  http://www.life.uiuc.edu/belmont/dietzel

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