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March 2000

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Subject:
Visible DNA stain.
From:
waldir Caldeira <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 21 Mar 2000 10:19:24 -0300
Content-Type:
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    Hello.

    I work with  insects gut epithelium  and   I have had the same
problem with PI, ToTo and BoBo, that stain all  nuclei and cytoplasm.
    Would it be possible for someone to tell me which concentration of
RNAase I should use in order to avoid inespecific staining?
    Thanks.


Waldir Caldeira
IBUSP
SÃO PAULO/BRASIL

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