Dear Dr. Taylor,

I can recommend you the dye congo red which can be excited with the 488 or
514 nm laser lines. The dichroic properties of the dye can be used to
visualize the mean cellulose microfibril orientation in plant cell walls
as discussed in 

Verbelen and Stickens (1995) In vivo determination of fibril orientation
in plant cell walls with polarization CLSM. J Microsc. 177, 1-6 

Verbelen and Kerstens (2000) Polarization confocal microscopy and congo
red fluorescence: a simple and rapid method to determine the mean
cellulose firbil orientation in plants. J. Microsc. 198, 101-107.

Best wishes,


Kris

---------------------------------------------------------------------------
Kris Vissenberg                       _/      _/    _/     _/_/_/_/
PhD-Student                          _/      _/          _/      _/
Dept.Biology (C 0.24)               _/      _/    _/    _/      _/
Plant Physiology / Morphology      _/      _/    _/    _/      _/
University of Antwerp (UIA)       _/      _/    _/    _/_/_/_/_/
Universiteitsplein 1             _/      _/    _/    _/      _/
B - 2610 Wilrijk (Antwerpen)    _/      _/    _/    _/      _/
Belgium                          _/_/_/_/    _/    _/      _/

Phone :+32-3-820.22.58
Fax n°:+32-3-820.22.71
E-mail:[log in to unmask]
---------------------------------------------------------------------------