CONFOCALMICROSCOPY Archives

June 2000

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Lloyd Donaldson <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 13 Jun 2000 08:34:53 +1200
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Joachim

On our Leica TCS/NT there is a table in the help file which details the optimum
pinhole size for each lens. If you use a pinhole setting of 1 then the size can
be found from the table. Unfortunately if you use a setting other than 1 I have
no idea how this relates to the actual size. It is logical to assume that a
setting of say 0.9 means 0.9 of the value at a setting of 1 but you would have
to check with Leica in case it is a non linear scale.
Hope that helps

Lloyd Donaldson
Forest Research, Rotorua, New Zealand

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