CONFOCALMICROSCOPY Archives

August 2000

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Subject:
HeNe 633 (was yellow vs red HeNe)
From:
Nicholas Terry <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 31 Aug 2000 08:06:06 -0500
Content-Type:
text/plain
Parts/Attachments:
text/plain (23 lines) 
Rosemary asked

>What do people use the HeNe 633 laser for apart from Cy5?  If we got the
>HeNe 594 it would replace the 633.


To excite TOPRO-3 for nuclear staining. Allows use of a red channel for
some marker other than PI or EB while saving having to use a UV laser to
visualise Hoechst or DAPI.

Regards,
Nick


Nicholas Terry, M.A., Ph.D.,
Experimental Radiation Oncology - 066,
The University of Texas M. D. Anderson Cancer Center,
1515 Holcombe Blvd., Houston, Texas 77030.
'Phone: 713-792-3424,
'Fax: 713-794-5369.
http://drad52.mdacc.tmc.edu.
[log in to unmask]

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