We use a 633nm HeNe for studies of phycobilisomes in cyanobacteria.  We also
have a pulsed laser diode at around the same wavelength that has been used
for time resolved fluorescence measurements.

Mark Tobin
Daresbury Laboratory.


-----Original Message-----
From: Nicholas Terry [mailto:[log in to unmask]]
Sent: 31 August 2000 14:06
To: [log in to unmask]
Subject: HeNe 633 (was yellow vs red HeNe)


Rosemary asked

>What do people use the HeNe 633 laser for apart from Cy5?  If we got the
>HeNe 594 it would replace the 633.


To excite TOPRO-3 for nuclear staining. Allows use of a red channel for
some marker other than PI or EB while saving having to use a UV laser to
visualise Hoechst or DAPI.

Regards,
Nick


Nicholas Terry, M.A., Ph.D.,
Experimental Radiation Oncology - 066,
The University of Texas M. D. Anderson Cancer Center,
1515 Holcombe Blvd., Houston, Texas 77030.
'Phone: 713-792-3424,
'Fax: 713-794-5369.
http://drad52.mdacc.tmc.edu.
[log in to unmask]