We obtained similar subcellular patterns that co-localized with the Golgi (detected w/
antiGM130 antibodies).  Can we assume that your GFP-fusion protein is predicted to be
within/associated with an endomembranous organelle and that the negative GFP controls give
different cellular distributions?
Mill
--
Mill W. Miller, Ph. D.
Assistant Professor
Department of Biological Sciences
Wright State University
3640 Col. Glenn Hwy.
Dayton, OH 45435-0001

937.775.3215 (work phone)
937.775.2655 (dept. office phone)
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"Garfield, Susan (NCI)" wrote:

> Hello everyone,
>
> When transfecting a GFP fusion protein into NIH 3T3 cells (mouse
> fibroblasts), we are seeing distribution into large globular stuctures in
> the cell cytoplasm.  We have tried staining the lysosomes, but there is no
> colocalization.  Does anyone have any suggestions as to what these could be
> and of appropriate stains for colocalization?
>
>  <<Vadim SelK localization.doc>>
>
> Thanks,
>
> Susan Garfield
> Confocal Facility Manager
> Laboratory of Experimental Carcinogenesis
> Division of Basic Sciences
> National Cancer Institute, NIH
> Building 37, Room 3C28
> 37 Convent Drive MSC4255
> Bethesda, MD  20892-4255
> +1 (301)496-5688 x227 (voice); +1 (301)496-0734 (fax)
>
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>                                          Name: Vadim SelK localization.doc
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