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Date: | Tue, 27 Feb 2001 17:12:49 -0600 |
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Along this thread, does anybody out there put their Hg or Xe power
supplies on a line conditioner to improve the fluctuations or is the
line voltage the least of your worries. Tom
>From my observations of using both types of light source, the Xenon seems
>far more stable with time. You don't notice the fluctuations that you get
>with Hg bulbs. This is important for quantification, such as dual excitation
>ratio methods (eg. Fura-2).
>
>Stephen H. Cody,
>Colon Molecular and Cell Biology Laboratory,
>Ludwig Institute for Cancer Research,
>Post Office Royal Melbourne Hospital,
>Parkville, Victoria 3050, Australia.
>
>Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
>email: [log in to unmask]
>www.ludwig.edu.au/confocal
>
>
>> ----------
>> From: Tom Phillips[SMTP:[log in to unmask]]
>> Reply To: Confocal Microscopy List
>> Sent: Wednesday, 28 February 2001 2:08
>> To: [log in to unmask]
>> Subject: Re: Xenon lamps
>>
>> All true but I believe one disadvantage is the peak illumination is
>> less for some fluorochromes such as DAPI. We see a difference in our
> > two systems that have Hg or Xe.
> >
> >
--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)
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