Tom,
Line variation is a key problem. See earlier posting re: stabilized power
supplies.
Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MME-Microscopy.com/education
"Why didn't they teach us that sooner?" ... probably because no one
thought to call MME about customized, on-site courses. Offered in all
areas of microscopy, sample prep,and image analysis, they make an immediate
impact on your productivity.
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At 05:12 PM 2/27/01 -0600, Tom Phillips wrote:
>Along this thread, does anybody out there put their Hg or Xe power
>supplies on a line conditioner to improve the fluctuations or is the
>line voltage the least of your worries. Tom
>
>
>
> From my observations of using both types of light source, the Xenon seems
>>far more stable with time. You don't notice the fluctuations that you get
>>with Hg bulbs. This is important for quantification, such as dual excitation
>>ratio methods (eg. Fura-2).
>>
>>Stephen H. Cody,
>>Colon Molecular and Cell Biology Laboratory,
>>Ludwig Institute for Cancer Research,
>>Post Office Royal Melbourne Hospital,
>>Parkville, Victoria 3050, Australia.
>>
>>Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
>>email: [log in to unmask]
>>www.ludwig.edu.au/confocal
>>
>>
>>> ----------
>>> From: Tom Phillips[SMTP:[log in to unmask]]
>>> Reply To: Confocal Microscopy List
>>> Sent: Wednesday, 28 February 2001 2:08
>>> To: [log in to unmask]
>>> Subject: Re: Xenon lamps
>>>
>>> All true but I believe one disadvantage is the peak illumination is
>>> less for some fluorochromes such as DAPI. We see a difference in our
>> > two systems that have Hg or Xe.
>> >
>> >
>
>--
>Thomas E. Phillips, Ph.D.
>Associate Professor of Biological Sciences
>Director, Molecular Cytology Core Facility
>
>3 Tucker Hall
>Division of Biological Sciences
>University of Missouri
>Columbia, MO 65211-7400
>(573)-882-4712 (voice)
>(573)-882-0123 (fax)
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