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| Date: | Wed, 24 Oct 2001 15:38:20 -0500 |
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We are using a HeNe laser (543nm) with a newly installed power supply
(yesterday) and it is running at 100% transmission (using a Zeiss 510). T
>===== Original Message From Confocal Microscopy List
<[log in to unmask]> =====
>Hank
>
>We see this type of effect sometimes just prior to a laser failure [Kr/Ar].
>Do you find that the orientation or spacing changes when you increase or
>decrease the PMT voltage ?
>
>Regards
>Lloyd Donaldson - Microscopist
>Future Forests
>Forest Research, Rotorua, New Zealand
>Ph 64 7 343 5581
>email [log in to unmask]
>www.forestresearch.co.nz/mnz
>
>
>
> hpadams
> <[log in to unmask]> To:
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> Sent by: Confocal cc:
> Microscopy List Subject: moire
effects
> <[log in to unmask]
> FFALO.EDU>
>
>
> 25/10/01 09:01 AM
> Please respond to Confocal
> Microscopy List
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>
>
>
>
>Confocal listservers,
>I have a user who is using a 10x objective to image nerve tracks in brain
>tissue. He is using an aqueous mounting media which completely dries. On
>some
>preps he gets what looks like moire lines across his image. I have seen
>this
>before and thought it maybe caused by drying artifacts between coverslip
>and
>sections (which are picked up on the slide not the coverslip). Does anyone
>have any suggestions which precludes the formation of these moire? lines
>and
>an explanation of their formation. Also, they seem to be wavelenght
>dependent:
>more pronounced with the 543 line than the 488 line. Is there a relation
>with
>excitation or emission?
>
>Hank Adams
>Integrated Microscopy Core
>Molecular and Cellular Biology
>Baylor College of Medicine
>Houston, Tx
>
>Hank Adams
>Manager
>Integrated Microscopy Core
>Molecular and Cellular Biology
>Baylor College of Medicine
>Houston, Tx 77030
Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030
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