CONFOCALMICROSCOPY Archives

November 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Andrea Murmann <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 5 Nov 2001 21:24:08 -0600
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Hi all,

I am working on a LSM 510 (Zeiss) and I have been collecting 3 color
z-sections (fitc, rhod, cy5) in "multitrack" mode. I have noticed a
significant pixel shift between the green and red channel. Since I am
interested in showing colocalisation and want to use the 3D function of the
program to calculate a projection, this shift is irritating. How can I
compensate for the shift while in the LSM 510 program so that I do not have
to go picture by picture in Photoshop?

Thanks in advance,
Andi

_________________________________________________________

Andrea E. Murmann

The University of Chicago
Department of Medicine
Section Hematology/Oncology (Lab I-312)
5841 South Maryland Avenue, MC 2115
Chicago, IL 60637-1470
U.S.A.

fax:    (773) 702-3163
phone:  (773) 834-1539

e-mail: [log in to unmask]

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