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Dear list members,
I am currently measuring leaf cuticle thickness using the vertical section
images acquired from CLSM (either using reflection mode or after staining
with a lipophilic fluorescent dye). The problem I am having is that the
thickness values I get this way is far greater than those obtained with TEM
for the same plant species and growth stage. Does anyone have experience
with similar situations?
I would appreciate your ideas and comments.
Liu
====================
Zhiqian Liu, PhD
Scientist
Forest Research
Private Bag 3020
Rotorua, New Zealand
Tel: 64 (0)7 343 5886
Fax: 64 (0)7 343 5811