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Excitation/emission is as follows:
Tetracycline (440, 570/610) two excitation peaks
Calcein (495/520)
Xylenol orange (390/560)

Other bone dyes to consider are acidic fuchsin (540/630) and calcein blue
(375/435)

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Roy K. Kinoshita, Ph.D.
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Liz Nickless wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Can anyone help me with these chemicals?
>
> I need to know what the excitation and emission wavelengths are. I think
> the Calcein is probably Calcein Green so I know that one but I am not 100%
> sure of that. They have been used on some bone samples that someone has
> brought to me in the hope that we can visualise them with the Confocal. I
> can see the Calcein in the 510nm range but am not sure what I am getting in
> the second channel of my microscope it could be a combination of bith the
> Xylenol orange or the tetracycline???
>
> The samples have all been prepared some time ago so I don't think we can do
> anything about the preparation.
>
>   tetracycline hydrochloride (Supramycina
> pro infusione 500mg,
>   5mg/kg calcein (Calcein C-0875, Sigma Chemical
> xylenol orange
> (Xylenol Orange X-0127,
>
> CALCEIN IS BLUE OR GREEN, TETRACYCLINE-YELLOW,
>  >XYLENOL-ORANGE.
>
> I would very much appreciate any ideas
>
> Thanks
>
> Liz Nickless
>
> Liz Nickless
> Manager
> Confocal Microscope Facility
> Institute of Molecular BioSciences
> Massey University
> Palmerston North
> New Zealand