Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi
I note that the spectra for Tetracycline and Xylene Orange are probably
interchanged in the message below. Tetracycline is the UV excited dye.

Fluorescence spectra tables can be found at:
  http://www.fhcrc.org/shared_resources/imaging/Fluorochromes.html
  http://pingu.salk.edu/flow/fluo.html
and the searchable fluorochrome database at
  http://fluorescence.biorad.com/

cheers

Ian.

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Excitation/emission is as follows:
> Tetracycline (440, 570/610) two excitation peaks
> Calcein (495/520)
> Xylenol orange (390/560)
>
> Other bone dyes to consider are acidic fuchsin (540/630) and calcein blue
> (375/435)
>
> --
> Roy K. Kinoshita, Ph.D.
> Fluorescence Microscopy Product Manager
> Omega Optical, Inc.
> 210 Main Street
> Brattleboro, VT  05301
> Toll Free: (866) 488-1064
> Fax: (802) 254-3937 or (802) 254-6754
> www.omegafilters.com
> [log in to unmask]
>
> Liz Nickless wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Can anyone help me with these chemicals?
> >
> > I need to know what the excitation and emission wavelengths are. I think
> > the Calcein is probably Calcein Green so I know that one but I am not 100%
> > sure of that. They have been used on some bone samples that someone has
> > brought to me in the hope that we can visualise them with the Confocal. I
> > can see the Calcein in the 510nm range but am not sure what I am getting in
> > the second channel of my microscope it could be a combination of bith the
> > Xylenol orange or the tetracycline???
> >
> > The samples have all been prepared some time ago so I don't think we can do
> > anything about the preparation.
> >
> >   tetracycline hydrochloride (Supramycina
> > pro infusione 500mg,
> >   5mg/kg calcein (Calcein C-0875, Sigma Chemical
> > xylenol orange
> > (Xylenol Orange X-0127,
> >
> > CALCEIN IS BLUE OR GREEN, TETRACYCLINE-YELLOW,
> >  >XYLENOL-ORANGE.
> >
> > I would very much appreciate any ideas
> >
> > Thanks
> >
> > Liz Nickless
> >
> > Liz Nickless
> > Manager
> > Confocal Microscope Facility
> > Institute of Molecular BioSciences
> > Massey University
> > Palmerston North
> > New Zealand


--
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Dr Ian S. Harper, Director
Monash Micro Imaging
The Microscopy & Imaging Research Facility
Building 13C, Monash University,
Clayton, VIC 3800, Australia

Email: [log in to unmask]
Tel: +61 3 9905 5635;  Fax: +61 3 9905 2766
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