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Hi John,

With the Leica TCS SP2 software, you can do Image Separation and select the
images which you want to use for future manipulations. This is under the
processing software (Edit-Separation), however with the previous version it was
under 3D (Image Tool). A new file (including all the images in the edited stack)
is then generated called "Separation" and it is this file that you then use to
make projections, movies, etc.

With our Leica TCS 4D, we can manipulate the stack fairly easily using the
memory bar and then make the projection directly without having to generate
additional files..

Cheers,

Jacqui.

Jacqueline Ross
Biomedical Imaging Research Unit
Department of Anatomy with Radiology
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: 64 9 373 7599 Ext 7438
Fax: 64 9 373 7484

http://www.health.auckland.ac.nz/biru/



John Runions wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
>
> Just a general comment/question on this topic.  It seems to me that years
> ago I could simply go to the gallery of images within a Zeiss Z-series,
> select the portion of the series that I wanted to project and then project
> it.  Is this true?  The reason I ask is that that would seem to me to be a
> very useful feature.  I now use Leica confocal software and although I have
> mentioned this idea to several different engineers and sales people they
> have always just looked at me funnily (mind you, a lot of people do that) as
> if this had never occurred to them.   What I do now is manipulation of the
> image stack as has been described for Biorad stacks.  Is there a simpler way
> to do this with Leica software?
>
> John.
>
> -------
> C. John Runions, Ph. D.
> Department of Plant Sciences
> University of Cambridge
> Downing St.
> Cambridge UK
> CB2 3EA
>
> email: [log in to unmask]
> phone: (01223) 766 545
> http://www.plantsci.cam.ac.uk/Haseloff/JohnRunions/Home.html