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Since you apparently must keep these little embryos alive, perhaps the
following URL will help:

http://www.brineshrimpdirect.com/brineshrimpdirect-faq-1-2-13.html#bsdca

Regards,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail Drop:  Geology
West Chester University
West Chester, PA, 19383
http://darwin.wcupa.edu/casi/
Phone/FAX:  610-738-0437

-----Original Message-----
From: Julie A Reynolds [mailto:[log in to unmask]]
Sent: Thursday, April 03, 2003 9:58 AM
To: [log in to unmask]
Subject: Using confocal microscopy and MitoTracker to quantify
mitochondria


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Hello,

I am interested in estimating the volume density of mitochondria in brine
shrimp embryos. The shell around the encysted embryos makes it difficult to
fix the embryos for EM, so I am looking for alternative methods. Somebody
in my lab suggested that I squish the embryos, label the mitochondria with
MitoTracker and use a point counting method to estimate # of mitochondria
in images taken with a confocal microscope.

Has anyone else tried anything remotely like this? If so do you have any
words of wisdom? So far I am having only limited success with this
technique.


Any assistance will be greatly appreciated.

Julie


Julie Reynolds
Graduate Research Assistant
Dept. of Biological Sciences
Louisiana State University
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