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Dear Pedro

This can be due to the scanning procedure in the 1024. At low zoom the
scanning system has an unreaded space between  two lines. This can be seen
by the fact that increasing the zoom will not increase the bleaching rate
proportionally. It means that the real width of the scanning laser beam is
much smaller than the width of a scanning line. The result is that part of
your sample will not be excited and  hence is not bleached.

Bye

Patrick

----- Original Message -----
From: "Dr Pedro J Camello" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, April 07, 2003 2:19 PM
Subject: Zoom problem


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
>
> I´m using a BioRad 1024 equipped with a 100 mW Ar laser. While imaging
> Ca2+ signals of fluo-4-loaded smooth muscle cells we found that using a
> 3x digital zoom we were unable to get even small responses. Reducing
> the zoom below 2x resolved the problem, and cells started to give perfect
> responses. We did not see big fluorescence bleaching
>
> Any explanations? :(
>
>
>
> Dr Pedro J Camello
> Dept Physiology
> Fac Veterinary Sci
> Univ of Extremadura
> Campus Universitario
> 10071 Caceres
> SPAIN
> Phone/Fax: 927-25-71-54
> [log in to unmask]
>
>