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As a imaging software Vendor we use Metamorph or Metavue, they have an
Equalize function.  Briefly the function can take the average, minimum, or
maximum light intensity in each plane, or an ROI in an image stack set it to
equal the average, minimum, or maximum intensity, respectively.

Scott Monroe, Ph.D.
Nikon Instruments Inc.

----- Original Message -----
From: "Tobias" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, April 08, 2003 11:18 AM
Subject: Re: How to Normalize Image Series


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> > Can anyone recommend a software or method to normalize image
> > brightness for a confocal
> > image series?  We have been collecting large stacks of images (about
> > 100 images/stack)
> > and photo-bleaching progressively reduces the brightness of images.
> > We hope that we can
> > normalize these images before reconstructing them in 3D.
> >
>
> i use bleach_cor, part of the xcosm package. requires unix/linux
> system. but works quite fine for me. using the same algorithm
> (non-linear regression.. fairly straightforward i guess) should be
> possible to write a imagej plugin. anybody did already?
>
> tobias
>