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We have a few different systems all on inverted Zeiss microscopes. With
the big XL incubator we have quite some problems in keeping the
temperature stable. You need a second heating device for your cells. Two
of our systems have a small so called P-Insert, together with different
objective lens heating systems. One system has a lot of focus drift (it
takes 4-5 hours to stabalize), but the other system with a CO2 cover
stabalizes after 20 minutes and remaines very stable thereafter. I have
seen the same system on a Leica inverted scope with the same good
performance.

Hope this helps,
Gert van Cappellen
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http://www.eur.nl/fgg/endov/multiphton

Heather Edens wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>Our lab is looking at setting up a live cell imaging system for our
>confocal system.  If anyone could provide me with positive or negative
>feedback on systems they have running in their laboratory, I would be
>grateful.  We have a Zeiss 510 Meta system with both a standard and
>inverted microscope and heated stage.  We want to image neutrophils
>migrating through a monolayer of epithelial cells that are grown on the
>underside of a permeable support.  The neutrophils are applied to the
>top of the support, they migrate through the filter and then through the
>
>epithelium in the basolateral to apical direction.  After migrating, the
>
>neutrophils would fall down into a lower reservoir.  Thus, having the
>epithelium grown below the filter makes the system a little bit more
>tricky.
>Thank you for your advice,
>Heather Edens
>Emory University
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>
>