Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I'll also offer my support for Autodeblur - we use it intensively for
correcting z-axis distortion in high magnification confocal stacks.
We've calibrated the corrected images against TEM data so we are pretty
sure that the blind deconvolution is giving us the appropriate results.
I haven't used other deconvolution packages, so I can't comment on the
relevant benefits of them, but it is a real bonus not having to measure
/ calculate a point spread function...
IAN
On Tuesday, October 28, 2003, at 02:14 AM, Mayandi Sivaguru wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hai, After my experience with the Autoquant/autodeblur programs at UBC
> Vancouver, I am continuously using this program to deconvolve our
> widefield
> and confocal images routinely (both fluorescence and bright field) and
> then
> using Imaris for rendering.
> In my experience, Autoquant's Sperical aberration correction and
> deconvolution package is a well designed program provides exceptional
> clarity to images, otherwise obscure even out of the best confocal
> setting.
> It has a great help book (available as a pdf) built in the program
> provides
> state of the art and accurate information of basic image acquisition
> and
> analysis. You can download the entire program free from their website
> and
> use it for 30 days and you will understand by yourself.
> Further, it supports all confocal and most wide field system producers
> native file format at any bit depth, including Imaris image analysis
> program which will enable you to open your stacks and individual images
> with a single click. It is really awesome. If you need any other info,
> please do not hesitate to call me or contact me offline.
>
> --No commercial interest/affiliation with any of the manufactures
> listed
> above--
> Shiv
>
> Mayandi Sivaguru, Ph.D.,
> Associate Director
> Molecular Cytology Core Facility
> Molecular Biology Program
> 2, Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> [log in to unmask]
>
> Voice: 1-573-882-4895
> Fax: 1-573-882-0123
>
> www.biotech.missouri.edu/mcc/
>
>
> At 06:02 PM 10/27/03 -0500, you wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> We are interested in deconvolution packages and seeking expert advice.
>> Since we mainly use Imaris, any comments/suggestion on AutoDeblur by
>> Bitplane / AutoQuant Imaging are particularly appreciated. Thanks in
>> advance.
>>
>> Hai Qi, M.D., Ph.D.
>> Laboratory of Immunology, NIAID
>> National Institute of Health
>>
>> TEL: 301-4961979
>> EMAIL: [log in to unmask]
>
>
Prof Ian Gibbins
Anatomy & Histology
Flinders University
GPO Box 2100
Adelaide SA 5001
AUSTRALIA
[log in to unmask]
voice: +61-8-8204 5271
fax: +61-8-8277 0085
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