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"If the slider moves erratically, it is going to result in jaggies.
Before we began any spectral work on unknowns, we looked at known
samples
of FITC, Dapi, etc.Mike "
I use the Chroma slide as a test slide for generating a spectra. The
spectra for the PMTs should be the same with regard to peak height and
trailing edge and forward edge. The only variable that should influence
the data should be the quality of the PMT.
Bob
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory
Reproductive Toxicology Division, MD 72
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]
Michael Cammer
<[log in to unmask] To: Robert Zucker/RTP/USEPA/US@EPA
edu> cc:
Subject: Re: Spectral Problems
11/19/03 05:19 PM
If the slider moves erratically, it is going to result in jaggies.
Before we began any spectral work on unknowns, we looked at known
samples
of FITC, Dapi, etc.
At 05:06 PM 11/19/2003 -0500, you wrote:
>Mike
>I tend to agree with your assessment ( slider is defective) as the
>system used to work prior to Leica replacing a slider. What I do not
>understand is how this happens. What is more disturbing is the
>possibility that incorrect spectra will be achieved by this mechanical
>defect. Since the Leica has three different channels and we can use
the
>same dichroic or reflector, the only difference is in the quality of
the
>PMT. That should not be that great so we can actually measure the
>optical clarity of the system. In this case we observed spectra with
>jagged edges in one of the PMT. We could have misinterpreted the data
to
>mean that we had some really unique spectra on a biological sample and
>more frightening published those results as a great observation.. I
>guess this means that we now need a test to make sure our sliders are
>working properly.
>Thanks for your input.
>Bob
>
>Robert M. Zucker, PhD
>U.S. Environmental Protection Agency
>Office of Research and Development
>National Health and Environmental Effects Research Laboratory
>Reproductive Toxicology Division, MD 72
>Research Triangle Park, North Carolina, 27711
>Tel: 919-541-1585; fax 919-541-4017
>e-mail: [log in to unmask]
>
>
>
>
>
> Michael
> Cammer
>
> <[log in to unmask] To: Robert
> Zucker/RTP/USEPA/US@EPA
> edu> cc:
>
> Subject: Re: Spectral
> Problems
> 11/19/03 07:23
> AM
>
>
>
>
>
>
>
>
>spectra of the PMTs are different according to our service rep. but
>this
>shouldn;t be the problem; I think the slits aren't stepping properly
and
>you need to have the mechanism fixed.
>
>
>___________________________________
>WORK: http://www.aecom.yu.edu/aif/
>PERSONAL: http://www.art-studio.us/
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of
Med.
Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY
10461
(718) 430-2890 Fax: 430-8996 URL:
http://www.aecom.yu.edu/aif/
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