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Hi Daniel,
it is my opinion that the best commercial deconvolution software is
Huygens (www.svi.nl). It is also possible to download XCOSM for free.
IN both cases you have to deal with installation, machine power, some
expertise etc...
In my LAB (www.lambs.it...still in progress but with some useful
information) we decided to realize a web based service that is for free
and that you can find at www.powermicroscope.com.
You simply have to send your images thorugh the web waiting for an
e-mail that tells you when they are ready (we need more or less half a
day depending on traffick.
Our program is completely automatic and this is the reason why for some
periods it did not work: 1) failure of the computer where it is
installed; 2) strange or funny image formats.
I do invite anyone to use it. I'd appreciate a feedback.
Software has been designed, written and implemented by a group of
seniors and juniors (that did the dirty hard job!), namely: ALberto
Diaspro, Patrizia Boccacci, Mario Bertero, Paola Bonetto, Giuseppe
Vicidomini, Marco Scarito, Mattia Epifani, Mirko Davolio, Francesco Di
Fato.
All my best
Alby
On Giovedė, mar 11, 2004, at 23:14 Europe/Rome, Daniel Amiot wrote:
> Greetings!
>
> I am interested in deconvoluting my confocal Z-stacks. I have done
> deconvolution before, but only on epifluorescence microscope images.
> I am using a Zeiss PASCAL system to create files with *.lsm
> extensions. What is the best software to use for this deconvolution
> for confocal images? And what shall I look for to differentiate
> confocal vs. epifluorescence deconvolution?
>
> Daniel M. Amiot II
> Department of Hypertension
> Case-Western Reserve University
> [log in to unmask]
>
........................................................................
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... non basta, resistere, resistere...resistere
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Alberto Diaspro
Department of Physics, University of Genoa
Via Dodecaneso 33, 16146 Genoa, Italy
voice: +39-0103536426/480 fax 010314218
[log in to unmask], http://www.lambs.it
http://wiley.com/cda/product/0,,0471409200,00.html
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